Generation of red fluorescent protein transgenic dogs |
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| Authors: | So Gun Hong Min Kyu Kim Goo Jang Hyun Ju Oh Jung Eun Park Jung Taek Kang Ok Jae Koo Teoan Kim Mo Sun Kwon Bon Chul Koo Jeong Chan Ra Dae Yong Kim CheMyong Ko Byeong Chun Lee |
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| Affiliation: | 1. College of Veterinary Medicine, Seoul National University, Seoul, Korea;2. College of Agriculture and Life Sciences, Chungnam National University, Daejeon, Korea;3. Catholic University of Daegu School of Medicine, Daegu, Korea;4. RNL Bio Co., Ltd., Seoul, Korea;5. Department of Clinical Sciences, University of Kentucky, Lexington, Kentucky |
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| Abstract: | Dogs (Canis familiaris) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein (RFP) gene‐expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP‐fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP. genesis 47:314–322, 2009. © 2009 Wiley‐Liss, Inc. |
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| Keywords: | dog transgenic fetal fibroblasts nuclear transfer red fluorescent protein |
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