Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes |
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| Authors: | Adriano Spreafico Federico Chellini Bruno Frediani Giulia Bernardini Silvia Niccolini Tommaso Serchi Giulia Collodel Alessandro Paffetti Vittorio Fossombroni Mauro Galeazzi Roberto Marcolongo Annalisa Santucci |
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| Affiliation: | 1. Sezione di Reumatologia, Dipartimento di Medicina Clinica e Scienze Immunologiche, Università di Siena, Policlinico Le Scotte, Siena, Italy;2. Centro Interdipartimentale per lo Studio Biochimico e Clinico delle Patologie Osteoarticolari, Università di Siena, Siena, Italy;3. Dipartimento di Biologia Molecolare, Università di Siena, Via Fiorentina 1, Siena, Italy;4. Dipartimento di Scienze Biomediche, Università di Siena, Policlinico Le Scotte, Siena, Italy;5. U.O.C. Immunoematologia Trasfusionale, Azienda Ospedaliera Universitaria Senese, Siena, Italy |
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| Abstract: | The aim of the present study was to demonstrate the mitogenic and differentiating properties of platelet‐rich plasma releasates (PRPr) on human chondrocytes in mono‐ and three‐dimensional cultures. In order to assess if PRPr supplementation could maintain the chondrocyte phenotype or at least inhibit the cell de‐differentiation even after several days in culture, we performed a proteomic study on several cell cultures independently grown, for different periods of time, in culture medium with FCS, human serum (HS), and releasates obtained from PRP and platelet‐poor plasma (PPP). We found that PRP treatment actually induced in chondrocytes the expression of proteins (some of which novel) involved in differentiation. J. Cell. Biochem. 108: 1153–1165, 2009. © 2009 Wiley‐Liss, Inc. |
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| Keywords: | platelet rich plasma chondrocyte growth factors proteome tissue engineering |
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