Swiprosin‐1 is expressed in mast cells and up‐regulated through the protein kinase CβI/η pathway |
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Authors: | Ramesh P. Thylur Young‐Dae Kim Min‐Sung Kwon Hyun‐Mee Oh Ho‐Keun Kwon Sang‐Hyun Kim Sin‐Hyeog Im Jang‐Soo Chun Zee‐Yong Park Chang‐Duk Jun |
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Affiliation: | 1. Department of Life Science, Cell Dynamics Research Center, BioImaging Research Center, and Research Center for Biomolecular Nanotechnology, GIST, Gwangju 500‐712, South Korea;2. Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700‐422, South Korea |
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Abstract: | Swiprosin‐1 exhibits the highest expression in CD8+ T cells and immature B cells and has been thought to play a role in lymphocyte physiology. Here we report that swiprosin‐1 is also expressed in mast cells and up‐regulated in both in vitro cultured mast cells by phorbol ester and in vivo model tissues of passive cutaneous anaphylaxis and atopic dermatitis. Targeted inhibition of the specific protein kinase C (PKC) isotypes by siRNA revealed that PKC‐βI/η are involved in the expression of swiprosin‐1 in the human mast cell line HMC‐1. In contrast, down‐regulation of swiprosin‐1 by A23187 or ionomycin suggests that calcium‐signaling plays a negative role. The ectopic expression of swiprosin‐1 augmented PMA/A23187‐induced NF‐κB promoter activity, and resulted in increased expression of cytokines. Moreover, knock‐down of swiprosin‐1 attenuated PMA/A23187‐induced cytokine expression. Collectively, these results suggest that swiprosin‐1 is a PKC‐βI/η‐inducible gene and it modulates mast cell activation through NF‐κB‐dependent pathway. J. Cell. Biochem. 108: 705–715, 2009. © 2009 Wiley‐Liss, Inc. |
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Keywords: | mast cells swiprosin‐1 protein kinase C calcium |
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