Efficient production of androgenetic embryos by round spermatid injection |
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Authors: | Hiromi Miki Michiko Hirose Narumi Ogonuki Kimiko Inoue Fuyuko Kezuka Arata Honda Kazuyuki Mekada Ken‐Ichi Hanaki Hirotaka Iwafune Atsushi Yoshiki Fumitoshi Ishino Atsuo Ogura |
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Institution: | 1. RIKEN Bioresource Center, Tsukuba, Ibaraki, Japan;2. Graduate School of Life and Environmental Science, University of Tsukuba, Tsukuba, Ibaraki, Japan;3. The Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Bunkyo‐ku, Tokyo, Japan;4. Medical Research Institute, Tokyo Medical and Dental University, Chiyoda‐ku, Tokyo, Japan |
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Abstract: | Mammalian androgenetic embryos can be produced by pronuclear exchange of fertilized oocytes or by dispermic in vitro fertilization of enucleated oocytes. Here, we report a new technique for producing mouse androgenetic embryos by injection of two round spermatid nuclei into oocytes, followed by female chromosome removal. We found that injection of round spermatids resulted in high rates of oocyte survival (88%). Androgenetic embryos thus produced developed into mid‐gestation fetuses at various rates, depending on the mouse strain used. All the fetuses examined maintained paternally specific genomic imprinting memories. This technique also enabled us to produce complete heterozygous F1 embryos by injecting two spermatids from different strains. The best rate of fetal survival (12% per embryos transferred) was obtained with C57BL/6 × DBA/2 androgenetic embryos. We also generated embryonic stem cell lines efficiently with the genotype of Mus musculus domesticus × M. m. molossinus. Thus, injection of two round spermatid nuclei followed by maternal enucleation is an effective alternative method of producing androgenetic embryos that consistently develop into blastocysts and mid‐gestation fetuses. genesis 47:155–160, 2009. © 2009 Wiley‐Liss, Inc. |
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Keywords: | oocyte ICSI ROSI androgenesis fetus embryo |
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