Selective targeting of green fluorescent nanodiamond conjugates to mitochondria in HeLa cells |
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| Authors: | Msaukiranji Mkandawire Andrea Pohl Tatiana Gubarevich Victoria Lapina Dietmar Appelhans Gerhard Rödel Wolfgang Pompe Jürgen Schreiber Jörg Opitz |
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| Institution: | 1. Institute of Genetics, University of Technology Dresden, Zellescher Weg 20b, 01217 Dresden, Germany;2. Institute of Materials Science, University of Technology Dresden, Hallwachsstra?e 03, 01069 Dresden, Germany;3. Fraunhofer‐Institute for Non‐Destructive Testing, Maria‐Reiche‐Stra?e 2, 01109 Dresden, Germany;4. Stepanov Institute of Physics, Academy of Science of Belarus, 68 Independence Avenue, Minsk BY‐220072, Republic of Belarus;5. Leibniz Institute of Polymer Research Dresden, Hohe Stra?e 6, 01069 Dresden, Germany;6. The Max‐Bergmann‐Center of Biomaterials Dresden, Budapester Stra?e 27, 01069 Dresden, Germany |
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| Abstract: | Fluorescent cellular biomarkers play a prominent role in biosciences. Most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. In view of this, we investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanodiamonds were functionalized by attaching antibodies that target intracellular structures such as actin filaments and mitochondria. Then, the nanodiamond conjugates were transfected into HeLa cells. Transfections were mediated by 4th‐generation dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful transfections of the nanodiamonds into HeLa cells. Nanodiamond fluorescence could be easily differentiated from cellular autofluorescence. Furthermore, nanodiamonds could be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for intracellular assays. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | ultradispersed diamonds (UDD) UDD immunoconjugates fluorescent biomarkers biofunctionalization antibodies transfection glycodendrimers |
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