A cyp19a1b‐gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells |
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| Authors: | Sok‐Keng Tong Karen Mouriec Ming‐Wei Kuo Elisabeth Pellegrini Marie‐Madeleine Gueguen François Brion Olivier Kah Bon‐chu Chung |
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| Affiliation: | 1. Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan;2. Institute of Biochemistry and Molecular Biology, National Yang‐Ming University, Taipei, Taiwan;3. Neurogenesis and ?stradiol, Université de Rennes 1, UMR CNRS 6026, Rennes, France;4. INERIS, Direction des Risques Chroniques, Unité d'évaluation des Risques écotoxicologiques, Verneuil‐en‐Halatte, France |
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| Abstract: | Aromatase is an enzyme that catalyzes the synthesis of estrogen in gonads and brain. Teleost fish express aromatase (AroB) strongly in the brain facilitating its detailed examination. To understand the function of AroB in the brain, we generated transgenic zebrafish that expresses green fluorescent protein (GFP) driven by the brain aromatase cyp19a1b promoter. GFP was found in the radial glial cells of transgenic larvae and adult fish that overlap with AroB immunoreactivity in the correct temporal and spatial pattern. GFP was also coexpressed with radial cell marker BLBP, but was not in neurons. In addition, GFP expression in the radial glial cells was stimulated by estrogen, same as endogenous AroB expression. Thus, this transgenic line faithfully mimics the regulation of AroB expression in radial glial cells. It provides a powerful tool to further characterize progenitor radial cells in adult and developing fish and to evaluate estrogenic activities of xenoestrogens and phytoestrogens. genesis 47:67–73, 2009. © 2008 Wiley‐Liss, Inc. |
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| Keywords: | aromatase estrogen synthase estradiol estrogen receptors radial glia brain lipid binding protein transgenic development zebrafish GFP brain central nervous system |
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