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Amyloid precursor protein expression is induced by tumor necrosis factor α in 3T3‐L1 adipocytes
Authors:Grit Sommer  Susan Kralisch  Jana Lipfert  Sebastian Weise  Kerstin Krause  Beate Jessnitzer  Ulrike Lössner  Matthias Blüher  Michael Stumvoll  Mathias Fasshauer
Institution:Department of Internal Medicine III, University of Leipzig, 04103 Leipzig, Germany
Abstract:Amyloid precursor protein (APP) has been characterized as an adipocyte‐secreted protein that might contribute to obesity‐related insulin resistance, inflammation, and dementia. In the current study, regulation of APP by the proinflammatory and insulin resistance‐inducing cytokine tumor necrosis factor (TNF) α was determined in 3T3‐L1 adipocytes. Interestingly, APP protein synthesis and mRNA expression were significantly increased by TNFα in a time‐dependent manner with maximal induction observed after 24 h of treatment. Furthermore, TNFα induced APP mRNA expression dose‐dependently with maximal 6.4‐fold upregulation seen at 100 ng/ml effector. Moreover, inhibitor experiments suggested that TNFα‐induced APP expression was mediated by nuclear factor κ B. Taken together, we show for the first time a potent upregulation of APP by TNFα suggesting a potential role of this adipocyte‐secreted protein in TNFα‐induced insulin resistance and inflammatory disease. J. Cell. Biochem. 108: 1418–1422, 2009. © 2009 Wiley‐Liss, Inc.
Keywords:3T3‐L1 adipocyte  adipokine  amyloid precursor protein  TNFα  
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