New matrices for the purification of pectinases by affinity chromatography |
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| Authors: | J Lobarzewski J Fiedurek G Ginalska T Wolski |
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| Institution: | 1. College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea;2. School of Pharmacy, Yantai University, Yantai 264005, PR China;3. KEMIMEDI Inc., Seoul 06106, Republic of Korea;4. Food Science and Technology Major, Dong-Eui University, Busan 47340, Republic of Korea |
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| Abstract: | Polygalacturonic acid was used as a ligand in the affinity technique for pectinases purification from the filtrate of Aspergillus niger 71 culture. For this purpose four matrices were examined, namely, alkylamine controlled porous glass (CPG), alkylamine silica gel as well as keratin or polyamide coated silica gel. Good results of pectinase purification was obtained on silanized CPG or keratin coated silica gel supports. |
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