A simple method for maintaining high multiplication of Narcissus shoot cultures in vitro |
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| Authors: | Y. N. Chow C. Selby B. M. R. Harvey |
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| Affiliation: | (1) Department of Agricultural Botany, Queen's University of Belfast, Newforge Lane, Belfast, Northern Ireland, UK;(2) Agricultural Botany Research Division, Department of Agriculture for Northern Ireland, Newforge Lane, BT9 5PX Belfast, Northern Ireland, UK |
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| Abstract: | A simple method for stimulating and maintaining high in vitro multiplication of Narcissus shoot clump cultures was developed. Shoot clumps were subjected either to  normal cutting where leaves were trimmed to 20 mm in length at the beginning of each culture passage or to severe cutting where shoot clumps were cut down to the basal plate region removing all green tissue. Severe cutting at the beginning of each culture passage initially doubled the leaf multiplication, compared to normal cutting, but the difference between cutting treatments declined in successive passages. The improvement in leaf multiplication was maintained when shoot clumps were subjected to severe cutting only at every other culture passage, with no cutting in the alternate recovery passages.In vitro multiplication was increased by severe cutting in all seven Narcissus cultivars which were tested.Abbreviations NAA-1 naphthylacetic acid - BAP benzylaminopurine |
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| Keywords: | cultivar cutting in vitro multiplication Narcissus |
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