Kinetics of fluid-phase pinocytosis in Dictyostelium discoideum amoebae |
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| Authors: | G Klein M Satre |
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| Affiliation: | 1. School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland;2. National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland;3. Department of Academic Endocrinology, Beaumont Hospital, Beaumont Road, Dublin, Ireland;1. Harry Daly Museum and Richard Bailey Library, Australian Society of Anaesthetists, Sydney, Australia;2. Anesthesiology and Perioperative Medicine, Case Western Reserve University School of Medicine, 11100 Euclid Avenue, Cleveland, OH, 44106, USA;3. Case Western Reserve University School of Dental Medicine, 2124 Cornell Rd, Cleveland, OH, 44106, USA;4. Curator Emeritus and Laureate of the History of Anesthesia, American Society of Anesthesiologists’ Wood Library-Museum of Anesthesiology, 1061 American Lane, Schaumburg, IL, 60173-4973, USA;1. Department of Gynecology, Service de Gynécologie, Tours University Hospital, 2 Boulevard Tonnellé, Tours 37044, France;2. INSERM U1069 Université François-Rabelais, Tours, France;3. Department of Obstetrics and Gynecology, Hôpital Nord, APHM, Aix-Marseille University (AMU), Univ Avignon, CNRS, IRD, IMBE UMR 7263, 13397, Marseille, France;4. Department of Obstetrics and Gynecology, Centre Hospitalier Intercommunal, Creteil, France;5. Department of Gynecologic surgery, Jeanne de Flandre Hospital, CHRU LILLE, Rue Eugene Avinée 59037 Lille Cedex, France;6. Department of Surgical Oncology, Georges-Francois Leclerc Cancer Centre, Dijon, France;7. Department of Surgical Gynecology, Strasbourg University Hospital, Strasbourg, France;8. Department of Gynecology, Rennes University Hospital, France. INSERM 1242, COSS, Rennes. Université de Rennes 1. France;9. Department of Gynecologic and Oncologic Surgery and Obstetrics, Lyon Sud University Hospital, Hospices Civils de Lyon, Université Lyon 1, France;10. Department of gynecology, CHI Poissy-St-Germain, Université Versailles-Saint-Quentin en Yvelines, EA 7285 Risques cliniques et sécurité en santé des femmes, Université Versailles-Saint-Quentin en Yvelines, Versailles, France;11. Department of Gynecology, Bobigny University, AP-HP, Jean-Verdier Hospital, Bondy, France;12. Department of Gynecologic and Breast Surgery and Oncology, AP-HP, Pitié-Salpêtrière, University Hospital, Paris, France;13. Department of Gynecology and Obstetrics, Lariboisiere Hospital, 750019 Paris, France;14. Department of Gynecology and Obstetrics, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), Paris, France |
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| Abstract: | Kinetics of pinocytosis in Dictyostelium discoideum were investigated over an extended period of time (up to 6 hours) using fluorescein isothiocyanate (FITC)-dextran as a fluid-phase marker. FITC-dextran added to the medium accumulated rapidly inside the cells with a rate of influx equivalent to 9 microns3 of fluid/cell x min. After a period of about 90 min of uptake, the intracellular FITC-dextran level reached a plateau which corresponded to a strict balance between pinocytosis and exocytosis as shown both by efflux measurements and pulse experiments with (3H) dextran. At equilibrium, the amount of internalized marker reached a value equivalent to 790 microns3 of fluid taken up per amoeba, i.e. a volume paradoxically higher than the total aqueous space of the cell (520 microns3 ). FITC-dextran was thus markedly concentrated intracellularly. The endocytic compartment in which the intracellular FITC-dextran was concentrated could be completely washed out when FITC-dextran was removed from the external medium. |
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