Glycosphingolipids having blood-group ABH and Lewis specificities |
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| Authors: | S I Hakomori |
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| Affiliation: | 1. Department of Infectious Diseases and Immunology, University of Florida, Gainesville, FL, 32611, USA;2. Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Florida, Gainesville, FL, 32611, USA;3. Department of Comparative, Diagnostic and Population Medicine, University of Florida, Gainesville, FL, 32611, USA;4. Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, 32611, USA;5. Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA;6. Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA, 30322, USA;1. Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil;2. Laboratório de Cultivo Celular, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil;3. Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brazil;1. Murmansk State Technical University, 183010, Murmansk, Russia;2. Murmansk Marine Biological Institute, 183010, Murmansk, Russia;1. TransBIOTech, 201 Mgr Bourget, Lévis, Québec, Canada G6V 6Z3;2. PolyAnalytik Inc, Western Research Park, 700 Collip Circle, Suite 202, London, Ontario, Canada N6G 4X8;1. Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, D-48149, Münster, Germany;2. Institute for Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, D-38106, Braunschweig, Germany;3. Chemistry Division, Forest Research Institute, Dehradun, 248006, India |
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| Abstract: | - 1.(1)|All of the glycosphingolipids which carry blood-group ABH Lea and Leb specificities contain high content (10–15%) of fucose in addition to glucosamine, galactose, and glucose. Glycolipids with blood-group A, and A2 specificites contained N-acetylgalactosamine in addition to the mentioned components. In erythrocyte stromata, the presence of three types of A1 and two types of B glycolipids were noticed, while one active component (with the slowest migration rate on thin-layer chromatography) was not found in A2 erythrocytes.
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- 2.1.(a)|The glycolipid fraction with H and Leb activities was separated from human erythrocytes, while the H-active glycolipid without Leb activity was isolated from pancreas.
- 2.2.(b)|The Lea-active glycolipid and Leb-active glycolipid were separated from different cases of human adenocarcinoma and from various normal tissues such as pancreas, stomach, liver, spleen and kidney of group O individuals. The yields of these glycolipids from adenocarcinoma tissue and from normal glandular tissues (pancreas and stomach linings) were higher than those from erythrocyte stroma and from parenchymatous organs (spleen, kidney, liver).
- 3.(3)|Co-presence of both Lea- and Leb-glycolipids in the tissue of some individuals were noticed in glandular tissue and in adenocarcinoma tissue. The patterns of blood-group glycolipid in adenocarcinoma tissue was distinguishable from that of normal glandular tissue by the absence of blood-group A- or B-glycolipid.
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