Biosynthesis of riboflavine by a purine-requiring mutant strain of Escherichia coli |
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Authors: | D J Howells and G W E Plaut |
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Institution: | Laboratory for the Study of Hereditary and Metabolic Disorders and Departments of Biological Chemistry and Medicine, University of Utah College of Medicine, Salt Lake City, Utah, U.S.A. |
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Abstract: | 1. Riboflavine biosynthesis occurs in non-proliferating cultures of a purine-requiring strain of Escherichia coli (ATCC no. 13863). 2. No significant incorporation of radioactivity from 1-14C]glycine into either C-4a and C-9a of riboflavine or into nucleic acid purines is detected under the above conditions; appreciable incorporation of label into 5-aminoimidazole-4-carboxamide occurs. However, the label of 6-14C]guanine is incorporated significantly into C-4 of riboflavine and into nucleic acid adenine and guanine; the specific radioactivity of the riboflavine is approximately twice that of either adenine or guanine of nucleic acid. 3. These results show that a purine derivative is an obligatory intermediate in riboflavine biogenesis. |
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