Sequence of the gene encoding type F neurotoxin of Clostridium botulinum |
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Authors: | Alison K East PT Richardson D Allaway MD Collins TA Roberts Daphne E Thompson |
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Institution: | Department of Microbiology, AFRC Institute of Food Research, Reading, UK. |
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Abstract: | Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin determined. Analysis of the nucleotide sequence demonstrated the presence of an open frame encoding a protein of 1274 amino acids, similar to other botulinum neurotoxins. Upstream of the toxin gene is the end of an open reading frame which encodes the C-terminus of a protein with homology to non-toxic-non-hemagglutinin component of type C progenitor toxin. |
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Keywords: | Clostridium botulinum Type F neurotoxin Nucleotide sequence Polymerase chain reaction |
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