Prolactin controls branchial clcn2c but not atp1a1a.2 in zebrafish Danio rerio |
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Authors: | Jason P. Breves |
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Affiliation: | Department of Biology, Skidmore College, Saratoga Springs, New York, USA |
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Abstract: | This study examined the branchial epithelium of stenohaline zebrafish Danio rerio, and in particular Na+–Cl− cotransporter-like 2 (Slc12a10.2)-expressing ionocytes (Na+–Cl− cotransporter [Ncc]-cells), which mediate the active uptake of ions from freshwater environments. The study assessed whether the pituitary hormone prolactin (Prl) stimulates the expression of messenger (m)RNAs encoding a Clc Cl− channel family member (clcn2c) and a Na+–K+-ATPase α1 subunit (atp1a1a.2) expressed in Ncc-cells. Branchial clcn2c, but not atp1a1a.2 levels, were sensitive to Prl both in vitro and in vivo. These observations suggest that Prl contributes to maintaining systemic Cl− balance via the regulation of clcn2c. |
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Keywords: | Cl− channel gill ionocyte Na+–K+-ATPase prolactin zebrafish |
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