An unusual phosphodiesterase activity towardsP-nitrophenylphosphorylcholine present in rat brain membranes |
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| Authors: | J. N. Kanfer D. McCartney |
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| Affiliation: | (1) Department of Biochemistry, University of Manitoba, R3E 0W3 Winnipeg, Manitoba, Canada |
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| Abstract: | A phosphodiesterase activity present in rat brain membranes has been examined utilizing p-nitrophenylphosphorylcholine as the substrate. This enzyme activity has a pH optimum of 8.5, is stimulated by a variety of free fatty acids, requires either Zn+2 or Ca+2 and is relatively stable to heating at 75°C for 7.5 minutes. These properties appear to distinguish this particular activity from those previously reported for alkaline phosphatase, nonspecific phosphodiesterase, phosphodiesterases I and II, lecithinase, and sphingomyelinase. |
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| Keywords: | P-Nitrophenylphosphorylcholine heat stability fatty acid activatable |
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