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Protein slot blotting: An easy,rapid and reliable technique to identify the expression of a protein in transgenic plants |
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| Authors: | Alexandre de Kochko Rongda Qu Philippe Marmey Iann Rance Claude Fauquet Roger N. Beachy |
| Affiliation: | (1) Division of Plant Biology, The Scripps Research Institute, Iltab/orstom International Laboratory for Tropical Agricultural Biotechnology, 10666 North Torrey Pines Road, 92037 La Jolla, CA, USA;(2) Division of Plant Biology, The Scripps Research Institute, Iltab/tsri: International laboratory for Tropical Agricultural Biotechnology, 10666 North Torrey Pines Road, 92037 La Jolla, CA, USA |
| Abstract: | A technique based on immunological recognition of a foreign protein in transgenic plants has been developed. It allows a quick and reliable screening of many plant samples, improves the accuracy of the results compared to ELISA and is easier to carry out and more sensitive than a western immunoblot. This technique has also been tested to recognize foreign proteins in rice and tobacco leaf extracts. |
| Keywords: | Antibody protein transgenic plants transgene expression |
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