Variation of Cell-free Translation Profiles Among Pathogenic Strains of Soybean Mosaic Virus |
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| Authors: | L M Mansky DP Durand T B Bailey J H Hill |
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| Institution: | Departments of Plant Pathology, Microbiology, and Statistics, Iowa State University, Ames, Iowa, USA |
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| Abstract: | Cell-free translation products from isolates representing soybean mosaic virus (SMV) strains G1 to G7 and G7a, along with several other SMV isolates, were analyzed. SMV RNAs were translated in both rabbit reticulocyte lysates and wheat germ extracts, yielding approximately 20 translation products for each strain from each translation system. Comparison of translation profiles by the presence or absence of proteins allowed for the formation of distinctive groups from each cell-free translation system. Groupings formed by analysis of products from rabbit reticulocyte lysates correlated with pathogenicity; groupings formed by analysis of products from wheat germ extracts had no apparent biological significance. |
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