Influence of inorganic phosphate and organic buffers on cephalosporin production by Streptomyces clavuligerus |
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Authors: | Yair Aharonowitz Arnold L Demain |
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Institution: | (1) Department of Nutrition and Food Science, Massachusetts Institute of Technology, 02139 Cambridge, MA, USA;(2) Present address: Department of Microbiology, Tel-Aviv University, Tel-Aviv, Israel |
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Abstract: | A high concentration of potassium phosphate (75–100 mM) stabilized pH and supported extensive growth of Streptomyces clavuligerus in a chemically defined medium; such a concentration also inhibited cephalosporin production. Although Tris buffer was found to have detrimental effects on growth and antibiotic production, 3-(N-morpholino)-propane sulfonate (MOPS) or 2-(N-morpholino)-ethane sulfonate (MES) buffer provided a nontoxic buffering system. In the presence of MOPS buffer, cephalosporin production was optimal at 25 mM phosphate, whereas higher concentrations of phosphate progressively inhibited antibiotic production up to 85% without modifying the pH pattern. MOPS buffer can be used to conduct fermentations at a relatively constant pH value in shake flasks.List of Non-Common Abbreviations MOPS
3-(N-morpholino)propane sulfonic acid
- MES
2-(N-morpholino)ethane sulfonic acid |
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Keywords: | Phosphate Buffers Cephalosporin Streptomyces clavuligerus Cephamycin Antibiotic Fermentation Control pH |
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