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Lectin affinity chromatography |
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| Authors: | Iris West Owen Goldring |
| Institution: | (1) “White Hayes” Garden Close, Givons Grove, KT22 8LT Leatherhead, Surrey, UK |
| Abstract: | A protocol is described for the preparation of lectin affinity chromatography columns using purified lectins and preactivated matrices. A general method is given for the purification of glycoproteins on immobilized Con A. Methods for immobilizing Con A on CDI agarose, Affi-Gel 15, and carbonyl-diimidazole-activated agarose are described. |
| Keywords: | Con A Affinity chromatography lectin immobilization glycoprotein purification Affi-Gel 15 CDI agarose carbonyldiimidazole-activated agarose |
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