氯雷他定的生物信息学分析及其对COVID-19的潜在治疗意义

为探讨氯雷他定对新型冠状病毒肺炎的潜在治疗作用.从基因表达谱数据库(Gene expression omnibus,GEO)获取氯雷他定数据,分别进行差异分析、基因本体学(GO)富集分析、京都基因与基因组百科全书(KEGG)富集分析、蛋白质相互作用(Protein-protein interaction,PPI)网络分...

Bioinformatics analysis of loratadine and its potential therapeutic significance for COVID-19

To explore the potential therapeutic effect of loratadine on new coronavirus pneumonia (COVID-19), loratadine data was obtained from Gene Expression Omnibus (GEO). Differential analysis, gene ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, Protein-Protein Interaction (PPI) network analysis were conducted,and key genes were screened. Using the Epigenomic Precision Medicine Prediction Platform (EpiMed) established earlier by the research group for correlation analysis, the negatively related diseases, positive relevant drugs, and targets in vivo were screened out, and the literature of coronavirus and key targets in Chinese and English databases were studied for verification. Results show that a total of 642 differential genes were screened out, including 230 up-regulated and 412 down-regulated. Among them, most of the genes enriched in GO were involved in the processes of inflammatory reaction, hormone action, and metabolism of glycolipids. The KEGG pathway was mainly enriched with various viral infections, inflammatory mediators, complement and coagulation, digestive tract damage, arrhythmia, and oxidative stress. Combined with the prediction results of EpiMed, it was found that the effect of loratadine on the genome-wide expression profile was complex. In PPI, a total of 642 proteins were obtained, and 10 hub genes were selected, mainly related to nerve signal transduction, phosphorylation, inflammatory response, and other functions. EpiMed results indicate that loratadine may have potential therapeutic effects, including severe acute respiratory syndrome (SARS), influenza, community-acquired pneumonia, severe sepsis, respiratory syncytial virus infection, etc. Drugs that may have a therapeutic effect on COVID-19 were predicted, including etanercept, famciclovir, nevirapine, fluvastatin, chloroquine phosphate, polygonum cuspidatum, houttuynia cordata, forsythia, liquorice, and common anemarrhena rhizome. Through text mining, a total of 14 articles related to the role of coronaviruses and key targets were retrieved, which verified the effectiveness of the key targets. Therefore, loratadine can regulate heart regulation, immune response, oxidative stress, viral infections, and other processes or pathways. It may have anti-viral, immune regulation, and organ protection functions at the same time. The results have potential therapeutic significance for COVID-19 critically ill patients.