Ethanol metabolism in a peroxisome-deficient mutant of the yeast Hansenula polymorpha |
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Authors: | G.J. Sulter I.J. van der Klei J.P. Schanstra W. Harder M. Veenhuis |
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Affiliation: | Department of Microbiology, Biological Centre, Haren, The Netherlands |
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Abstract: | Abstract: This paper describes ethanol metabolism in a peroxisome-deficient (PER) mutant of Hansenula polymorpha . The PER mutant was able to use ethanol as sole-carbon source but showed reduced growth rates compared to wild-type cells together with a reduced rate of ethanol utilization under μmax conditions. In chemostat cultures at low-dilution rates, the activities of alcohol dehydrogenase, isocitrate lyase and malate synthase were comparable in wild-type and PER cells. In PER cells the two latter enzymes, exclusively microbody-bound in wild-type cells, were active in the cytosol. The possible advantage of intact microbodies in the intermediary metabolism of ethanol in H. polymorpha is discussed. |
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Keywords: | Hansenula polymorpha Peroxisome- Peroxisome-deficient mutant Glyoxylate cycle Ethanol metabolism |
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