A rapid procedure for purification of EcoRI endonuclease. |
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| Authors: | J Sümegi D Breedveld P Hossenlopp P Chambon |
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| Institution: | Laboratoire du CNRS de Biologie Moléculaire des Eucaryotes et Groupe de Recherche 44 de l''INSERM, Faculté de Médecine Strasbourg - France |
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| Abstract: | A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on phosphocellulose. The purified restriction endonuclease is free of exonuclease and other endonucleases. |
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| Keywords: | SV40 Simian Virus 40 |
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