Evaluation of regioselectivity of lipases based on synthesis reaction conducted with propyl alcohol, isopropyl alcohol and propylene glycol |
| |
Authors: | Pierrick Fevrier, Philippe Gu gan, Florent Yvergnaux, Jean Pierre Callegari, Laurent Dufoss ,Adrien Binet |
| |
Affiliation: | a PROFIL, Av. du Gal Leclerc, Campus de Beaulieu, 35700 Rennes, France b LUMAQ, EA 2651, Université de Bretagne Occidentale, 29000 Quimper, France |
| |
Abstract: | Preliminary investigations on the regioselectiviy of various lipases were performed. Ten commercial lipases from different origins, including three immobilized lipases, were tested by esterification reaction between caprylic acid and propyl or isopropyl alcohol in n-hexane. Reaction products were analyzed with a gas chromatograph. Best yields were obtained with immobilized lipase IM60 from Rhizomucor miehei. Therefore, this enzyme was chosen as biocatalyst for a second step of regioselectiviy study with propylene glycol which bears primary and secondary alcohol groups. It was shown, by using several solvents, that polarity could influence the product profile in situations in which multiple products of various polarities can be formed. Furthermore, the major role of silica gel in reaction mixture was established. |
| |
Keywords: | Lipase Regioselectivity Esterification Solvent polarity |
本文献已被 ScienceDirect 等数据库收录! |
|