| 雪花莲外源凝集素基因的克隆及序列分析 |
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| 引用本文： | 周岩 田颖川 陶慧中 莽克强. 雪花莲外源凝集素基因的克隆及序列分析[J]. 生物工程学报, 1996, 12(4) |
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| 作者姓名： | 周岩 田颖川 陶慧中 莽克强 |
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| 作者单位： | 中国科学院微生物研究所．植物生物技术开放实验室 北京 100080 |
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| 基金项目： | 国家863计划资助，国际科学文化中心(ICSC、瑞士.洛桑)世界实验室部分资助 |
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| 摘 要： | 外源凝集素(lectin)是自然界中广泛分布的一组蛋白质,在多种生物中均有发现。外源凝集素为一类能特异地识别并可逆结合糖类复合物的糖基部分而不改变被识别糖基的共价结构的非免疫性蛋白。它对植物有很重要的生理作用。例如保护功能,在植物生长的各个阶段以不同的方式保护植物免于害虫的侵害;作为储藏蛋白,在植物发芽和幼苗生长阶段,裂解的外源凝集素为其提供氨基酸;外源凝集素还可能参与细胞间的识别,如在植物建立共生关系中,根部的外源凝集素可能是宿主特异性的重要决定因素。
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Molecular Cloning and Sequence Analysis of Snowdrop (Galanthus nivalis) Lectin Gene |
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| Zhou Yan Tian Yingchuan Tao Huizhong Mang Keqiang. Molecular Cloning and Sequence Analysis of Snowdrop (Galanthus nivalis) Lectin Gene[J]. Chinese journal of biotechnology, 1996, 12(4) |
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| Authors: | Zhou Yan Tian Yingchuan Tao Huizhong Mang Keqiang |
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| Abstract: | Snowdrop lectin genes were cloned from DNA extracted from snowdrop young leaves by PCR. Sequence analysis revealed that the cloned gene GNA12 (Galan-thus nivalis agglutinin) which encode mature lectin and GNA34 which encode precursor lectin have a high homology with previously reported LECGNA2 gene. The sequence coding for mature lectin showed 97% and 98% homology at nucleotide and amino acid level respectively. However a 9-base direct repeat in the sequence coding for signal pep-tide of GNA34 makes it different from other previously reported LECGNA genes. |
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| Keywords: | Snowdrop (Galanthus nivalis L. )lectin molecular cloning sequence analysis |
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