Purification of inorganic pyrophosphatase from rat salivary glands |
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Authors: | M Koskowska-Dziadowicz M Ochman T Szymczyk |
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Institution: | Department of Biochemistry, Institute of Biopharmacy, Medical Academy in Warsaw, Poland. |
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Abstract: | Inorganic pyrophosphatase EC 3.6.1.1] isolated from rat sublingual and submandibular glands was purified 2300-fold and 2600-fold, respectively. The purified enzymatic preparations separated on electrophoresis into two protein bands, of which only one showed the pyrophosphatase activity. Inorganic pyrophosphatase from rat salivary glands is a monomeric anionic protein, its isoelectric point is 5.42 and 4.90 for the sublingual and submandibular glands, respectively. |
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