差异显示技术及其研究进展

差异显示技术是分离差异表达基因的重要方法与手段，同其它研究基因差异表达的方法如RDA，SSH，SAGE相比，差异显示技术是其中使用频率较高的方法。该技术自1992年创立以来，经过各国研究者不断完善与改进，现已大大克服以往诸多不足，扩大了应用领域。本文就差异显示技术的原理及主要优缺点作以简要概述，并着重就引物设计、降低假阳性、鉴定差异表达基因及差异显示技术的衍生技术这4个方面的研究进展作以详尽介绍。

Differential Display Technique and Its Progress

Differential display technique is an important method to isolate differentially expressed gene. Comparing to other methods like representational difference analysis, suppression subtractive hybridization and serial analysis of gene expression, differential display technique is used in higher frequency. Since it was established in 1992, it has overcome many disadvantages and widened its practical fields through improvements and enhancements by global researchers. In this paper the principle and the main advantages and disadvantages of differential display technique were briefly introduced. Meanwhile, the four progressed aspects in designing primer, reducing false positives, identifying differentially expressed gene and techniques derived from DD are introduced in detail.