Comparative immunochemical study of ribosomal proteins from various mammalian cells by two-dimensional immunoelectrophoresis |
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| Authors: | Marie-Annick Auger-Buendia Marie-Paule Ollier-Hartmann Armand Tavitian and Lucien Hartmann |
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| Institution: | (1) Département d'Oncologie Expérimentale, U.107 INSERM, LOI CNRS, Institut de Recherches sur les Maladies du Sang, Hôpital Saint-Louis, 2 place du Dr. A. Fournier, F-75475 Paris Cédex 10, France;(2) Laboratoire de Chimie Clinique et Biologie Moléculaire, ERA 696 CNRS, FRA 1 INSERM, Institut Biomédical des Cordeliers, 15 rue de l'Ecole de Médecine, F-75270 Paris Cédex 6, France |
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| Abstract: | Summary Proteins isolated from ribosomal subunits of various mammalian cells were analysed comparatively by two different methods: a two-dimensional polyacrylamide gel electrophoresis system and a recently described two-dimensional immunoelectrophoresis technique. For this purpose, antisera were raised in rabbits against the total mixture of ribosomal proteins from murine cells. These sera were characterized by ring-test, double immunodiffusion and two-dimensional immunoelectrophoresis. They were shown to contain antibodies to a large number of ribosomal proteins. Immunoelectrophoretic analysis of 60S and 40S subunit proteins from rabbit, lamb, canine and human cells using anti-murine sera revealed a striking conservation of their antigenic properties. These results corroborated those obtained by two-dimensional polyacrylamide gel electrophoresis. |
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