Histopathology of experimental Aspergillus fumigatus keratitis |
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Authors: | A. Fons J. García-de-Lomas J. M. Nogueira F. J. Buesa C. Gimeno |
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Affiliation: | (1) Departamento de Microbiología, Facultad de Medicina, Adva. Blasco Ibañez 17, E-46010 Valencia, Spain |
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Abstract: | Histopathological studies in rabbit's eyes, 7 and 14 days after intracorneal inoculation with 1×105Aspergillus fumigatus conidia have been performed.Similar lesions were found in both periods with fungal hyphae in the anterior third of corneal stroma, round cell infiltration from the sclero-corneal edge and in the anterior chamber and, neovascularization.No lesions were found in the Descemet's membrane.Gomori silver-methenamine stain with hematoxiline-eosine counter-stain was found to be the most reliable stain to detect fungal presence in corneal stroma, and Masson's trichromic stain in the study of pathological changes in ocular elements. |
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Keywords: | Aspergillus fumigatus keratitis histopathology experimental keratitis |
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