香菇锰过氧化物酶基因（LeMnP1）生物信息学分析及高温胁迫下的表达

本研究对香菇锰过氧化物酶基因（manganese peroxidase 1,MnP1）进行了生物信息学分析,选用香菇出发菌株18和诱变菌株18N44作为实验材料,经测定高温胁迫后菌丝恢复生长速度,分析了高温胁迫过程中香菇锰过氧化物酶基因（LeMnP1）的表达水平及酶活性。结果表明：LeMnP1定位于细胞外,该蛋白属于过氧化物酶超家族,与其他担子菌具有较高的同源性。18N44在高温胁迫后其恢复能力明显强于18;在高温胁迫过程中,18N44中LeMnP1的基因表达水平均高于18,呈现先上升后下降的趋势;在未处理时,18N44的MnP活性显著高于18,随着高温胁迫时间延长,其酶活性显著下降,18的LeMnP1基因表达量及酶活性在整个高温胁迫过程中基本维持稳定。据此推测锰过氧化物酶基因的相对高表达,可能是18N44高温胁迫后恢复生长快的原因之一。

Bioinformatics analysis of manganese peroxidase gene (LeMnP1) in Lentinula edodes and effects of high temperature stress on its expression

Bioinformatics analysis was performed of the manganese peroxidase 1 (MnP1) gene of Lentinula edodes. The strain 18 and its mutagenic strain 18N44 of L. edodes were selected as experimental materials, and gene expression and enzyme activity of manganese peroxidase during high temperature stress were determined. The results showed that LeMnP1 is located outside the cell. This protein belongs to the peroxidase superfamily and has high homology with that of basidiomycetes. The recovery ability of 18N44 was obviously higher than that of strain 18 after high temperature stress. During the high temperature treatment, the level of LeMnP1 gene expression in 18N44 was higher than that in 18, showing a tendency of first increasing and then decreasing. The MnP activity of 18N44 was significantly higher than that in 18 at 0h. With the prolongation of the duration of high temperature stress, MnP activity in 18N44 decreased significantly. However, LeMnP1 gene expression and enzyme activity remained stable in 18 during high temperature treatment. It is speculated that higher expression of LeMnP1 in 18N44 may be closely related to the faster recovery properties of 18N44 after high temperature stress.