磁场对金针菇菌丝生长的影响及磁受体和铁离子转运蛋白的鉴定表达

磁场作为一种无法避免的自然因子,对生物的生长发育有重要影响。然而,目前磁场对大型真菌影响的研究还鲜有报道。本研究采用铷磁铁对金针菇菌丝进行处理,检测金针菇菌丝在不同磁极刺激下的生长和基因表达规律。结果显示：磁场是铷磁铁抑制金针菇菌丝生长的主要原因,且在磁感应强度0.003T以上对菌丝生长的抑制作用较强。通过同源比对获得一个金针菇磁受体和一个铁离子转运蛋白的编码基因,分别命名为ff-magr和ff-fief。定量PCR结果显示ff-magr在N极和S极均出现显著下调表达,N极和S极磁场对ff-fief 的表达均无显著影响。结果表明：磁受体蛋白参与了金针菇菌丝受磁场抑制的应答过程,而磁受体不仅仅是通过调控铁的含量来调控菌丝的生长发育。以上结果为合理利用磁场调控真菌生长发育奠定基础,同时也为深入研究磁场对真菌生长发育的分子机理提供参考。

The effects of magnetic field on the growth of Flammulina filiformis and the identification and expression of magnetic receptor and iron ion transporter

As an inevitable natural factor, magnetic field has an important influence on the growth and development of organisms. However, there are few reports about the effect of magnetic field on large fungus growth. The growth and gene expression of Flammulina filiformis mycelium were detected by rubidium magnet treatment. The results showed that magnetic field of rubidium magnet inhibited the growth of Flammulina filiformis mycelium, and the inhibition was strong when the magnetic induction intensity was over 0.003T. A gene encoding the magnetic receptor named as ff-magr and an iron transporter ff-fief of Flammulina filiformis were obtained by homology matching. Quantitative PCR results showed that the expression of ff-magr was significantly down-regulated at both N and S poles, and the magnetic fields at both N and S poles had no significant effect on the expression of ff-fief, indicating that the magnetic receptor protein was involved in the response process of the inhibition of magnetic field, and the magnetic receptor not merely regulated the growth and development of mycelia by regulating iron content. The molecular mechanism of magnetic field influencing the growth and development of fungi needs further study.