裂褶菌菌株G18对孔雀石绿染料的脱色优化

通过对兔眼蓝莓幼果组织中分离得到的内生真菌G18进行形态特征、ITS序列和系统进化分析鉴定菌株G18为裂褶菌Schizophyllum commune。同时,对菌株G18产生的3种木质素降解酶进行监测,发现G18菌株可以分泌漆酶、木质素过氧化物酶和锰过氧化物酶。为明确裂褶菌G18对染料的脱色能力,利用裂褶菌G18对固体条件下8种染料进行脱色能力的检测,筛选出较易脱色的染料后,对该染料的脱色条件进行优化。结果表明,裂褶菌G18对8种染料均可以脱色,对孔雀石绿染料的脱色效果最好。裂褶菌G18对孔雀石绿的脱色优化结果为pH 7.0、20.0g/L淀粉、1.0g/L尿素、1.0g/L硫酸锌、接菌量9片（d=5.0mm）。

Optimization of decolorization of malachite green dye by Schizophyllum commune G18

The endophytic fungus G18 isolated from the young blueberry fruits of Vaccinium ashei was identified as Schizophyllum commune based on morphological characteristics, ITS sequence analysis and phylogenetic analysis. The lignin-degrading activity of three enzymes, laccase, lignin peroxidase and manganese peroxidase, produced by S. commune G18 were monitored base on decolorization ability of G18 to eight dyes. The results showed that the strain G18 could decolorize all the tested eight dyes under solid conditions, and decolorization effect on malachite green dyes was the best. The decolorization conditions of G18 to malachite green was optimized. The medium adjusted at pH 7.0 containing 20.0g/L starch, 1.0g/L urea, and 1.0g/L zinc sulfate was the best for obtaining the cultures with high decolorization ability. Inoculation quantity of 9 tablets (d=5.0mm) of inoculum seems suitable.