首页 | 本学科首页   官方微博 | 高级检索  
     

裂褶菌菌株G18对孔雀石绿染料的脱色优化
引用本文:尚晓静,张富美,程伟,苏莉,侯瑞. 裂褶菌菌株G18对孔雀石绿染料的脱色优化[J]. 菌物学报, 2020, 39(8): 1580-1592. DOI: 10.13346/j.mycosystema.200055
作者姓名:尚晓静  张富美  程伟  苏莉  侯瑞
作者单位:贵州大学林学院 贵州 贵阳 550025
基金项目:贵州省科技计划;国家自然科学基金
摘    要:通过对兔眼蓝莓幼果组织中分离得到的内生真菌G18进行形态特征、ITS序列和系统进化分析鉴定菌株G18为裂褶菌Schizophyllum commune。同时,对菌株G18产生的3种木质素降解酶进行监测,发现G18菌株可以分泌漆酶、木质素过氧化物酶和锰过氧化物酶。为明确裂褶菌G18对染料的脱色能力,利用裂褶菌G18对固体条件下8种染料进行脱色能力的检测,筛选出较易脱色的染料后,对该染料的脱色条件进行优化。结果表明,裂褶菌G18对8种染料均可以脱色,对孔雀石绿染料的脱色效果最好。裂褶菌G18对孔雀石绿的脱色优化结果为pH 7.0、20.0g/L淀粉、1.0g/L尿素、1.0g/L硫酸锌、接菌量9片(d=5.0mm)。

关 键 词:裂褶菌  白腐真菌  木质素降解酶  孔雀石绿  染料脱色  
收稿时间:2020-03-02

Optimization of decolorization of malachite green dye by Schizophyllum commune G18
Xiao-Jing SHANG,Fu-Mei ZHANG,Wei CHENG,Li SU,Rui HOU. Optimization of decolorization of malachite green dye by Schizophyllum commune G18[J]. Mycosystema, 2020, 39(8): 1580-1592. DOI: 10.13346/j.mycosystema.200055
Authors:Xiao-Jing SHANG  Fu-Mei ZHANG  Wei CHENG  Li SU  Rui HOU
Affiliation:College of Forestry, Guizhou University, Guiyang, Guizhou 550025, China
Abstract:The endophytic fungus G18 isolated from the young blueberry fruits of Vaccinium ashei was identified as Schizophyllum commune based on morphological characteristics, ITS sequence analysis and phylogenetic analysis. The lignin-degrading activity of three enzymes, laccase, lignin peroxidase and manganese peroxidase, produced by S. commune G18 were monitored base on decolorization ability of G18 to eight dyes. The results showed that the strain G18 could decolorize all the tested eight dyes under solid conditions, and decolorization effect on malachite green dyes was the best. The decolorization conditions of G18 to malachite green was optimized. The medium adjusted at pH 7.0 containing 20.0g/L starch, 1.0g/L urea, and 1.0g/L zinc sulfate was the best for obtaining the cultures with high decolorization ability. Inoculation quantity of 9 tablets (d=5.0mm) of inoculum seems suitable.
Keywords:Schizophyllum commune  white rot fungi  lignin degrading enzyme  malachite green  dye decolorization  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《菌物学报》浏览原始摘要信息
点击此处可从《菌物学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号