Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2 |
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Authors: | Fukuda Tomokazu Scott Gregory Komatsu Yoshihiro Araya Runa Kawano Masako Ray Manas K Yamada Masahisa Mishina Yuji |
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Institution: | Molecular Developmental Biology Group, Laboratory of Reproductive and Developmental Toxicology, 111 Alexander Drive, Research Triangle Park, NC 27709, USA. |
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Abstract: | BMP signaling plays pleiotropic roles in various tissues. Transgenic mouse lines that overexpress BMP signaling in a tissue-specific manner would be beneficial; however, production of each tissue-specific transgenic mouse line is labor-intensive. Here, using a Cre-loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre-expression mouse lines, Cre-mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. The conditional overexpression system described here provides versatility in investigating gene functions in a tissue-specific manner without having to generate independent tissue-specific transgenic lines. |
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Keywords: | Cre‐loxP system Smad transgenic mouse constitutively active bone morphogenetic protein |
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