Group-Specific Antibodies against Zearalenone and Its Metabolites and Synthetic Analogs |
| |
| Authors: | A. A. Burkin G. P. Kononenko N. A. Soboleva |
| |
| Affiliation: | (1) All-Russian Research Institute of Veterinary Sanitation, Hygiene, and Ecology of the Russian Academy of Agricultural Sciences, Moscow, 123022, Russia |
| |
| Abstract: | Formation kinetics, specificity, and analytical potential of polyclonal antibodies raised in rabbits against BSA conjugates of zearalanone carboxymethyloxime (CMO-ZAN) and zearalenone carboxymethyloxime (CMO-ZEN) have been studied. Preparation of the conjugates involved conversion of CMO-ZAN and CMO-ZEN into activated esters or carbodiimide condensation. Two versions of a group-specific enzyme immunoassay (for zearalenone/ -zearalenol and zearalanone/-zearalanol) based on the heterologous combination of solid-phase antigens are described (sensitivity, 0.01 ng/ml). |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
