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Aerobiological,biochemical and immunological studies on some of the dominant <Emphasis Type="Italic">Aspergillus</Emphasis> species of South Assam (India)
Authors:Dhruba Sharma  B K Dutta  A B Singh  B R Shome
Institution:(1) Microbiology Laboratory, Centre for Biodiversity, Department of Life Science, Rajiv Gandhi University, Itanagar, 791112, Arunachal Pradesh, India;(2) Department of Ecology and Environmental Science, Assam University, Silchar, 788011, Assam, India;(3) Institute of Genomics and Integrative Biology, CSIR, Delhi University Campus, Mall Road, Delhi, 110 007, India;(4) Division of Animal Health, ICAR Research Complex, Barapani, 793 103, Meghalaya, India
Abstract:Two years atmospheric survey of air-borne Aspergillus was carried out in the environmental conditions of South Assam. The survey revealed a total of 16 different species of Aspergillus with marked seasonal and annual variations. Aspergillus fumigatus was found to be the dominant atmospheric fungal species followed by Aspergillus flavus, Aspergillus niger, etc. Among the sample extracts tested, highest quantity of soluble protein was recorded in Aspergillus fumigatus (95.0 mg/g) whereas highest quantity of soluble carbohydrate (40.8 mg/g) and free amino acid (135.0 mg/g) was recorded in the sample extract of Aspergillus niger per gram of dry weight, respectively. The highest numbers of protein polypeptide bands were detected in the sample extract of Aspergillus fumigatus followed by Aspergillus flavus and lowest in Aspergillus niger. The maximum numbers of immunoglobulin E binding protein fractions were found in Aspergillus fumigatus, followed by Aspergillus flavus, Aspergillus clavatus, etc.
Keywords:Allergy            Aspergillus            ELISA  SDS-PAGE  Western blotting
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