Effects of carbon dioxide in anther cultures |
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Authors: | Lars Johansson Tage Eriksson |
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Institution: | Swedish Univ. of Agricultural Sciences, Dept. of Plant Breeding, P.O. Box 7003, S-75007 Uppsala, Sweden;Institute of Physiological Botany of the Univ. of Uppsala, Box 540, S-75121 Uppsala, Sweden. |
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Abstract: | In anther cultures of Anemone canadensis L., Anemone dichotoma L., Anemone hupehensis Lemoine, Clematis viticella L. and Papaver setigerum DC. a positive relationship between incubation in 2% CO2 and the production of microspore-derived embryos was observed. In anther cultures of Nicotiana tabacum L., Anemone hupehensis and Clematis viticella a combination of cold treatment (7°C) and incubation in 2% CO2 increased embryo production. In Anemone canadensis cold treatment increased the number of proembryos, whereas incubation in 2% CO2 had no effect. In Anemone hupehensis 5% CO2 increased embryo production by more than 2%. In Anemone dichotoma and Papaver setigerum 2% CO2 was the more efficient level. CO2 had no significant effect on pH in the culture medium in anther cultures of Anemone canadensis. |
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Keywords: | Anemone Clematis Nicotiana Papaver |
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