Inoculation and tissue distribution in pilot-scale plant root culture bioreactors |
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Authors: | Divakar Ramakrishnan John Salim Wayne R Curtis |
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Institution: | (1) Department of Chemical Engineering, The Pennsylvania State University, 16802 University Park, PA, USA;(2) The Biotechnology Institute The Pennsylvania State University, 16802 University Park, PA, USA;(3) 111 Fenske Laboratory, Dept. of Chemical Engineering, PSU, 16802 University Park, PA, USA |
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Abstract: | Summary Inoculation of large-scale plant root culture reactors can be carried out by briefly homogenizing bulk root tissue, followed by aseptic transfer as a slurry to the reactor. Uniform root distribution can be achieved in bioreactors by entrapment of the growing root inoculum onto process packing elements (e.g. distillation packings), randomly distributed within the reactor, in a bubble column operation. These two techniques have been successfully used to inoculate a 14 L pilot-scale reactor which is subsequently operated as a trickle bed reactor. |
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