Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph |
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Authors: | D Cullen S A Leong L J Wilson D J Henner |
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Institution: | Forest Products Laboratory, USDA, Madison, WI 53705. |
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Abstract: | Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to be associated with the A. nidulans pyrG89 allele. Plasmid pDH25 features a ClaI site immediately preceding the hph start codon thus permitting convenient replacement of the trpC sequences with other eukaryotic promoters. |
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