Pig genome analysis: differential distribution of SINE and LINE sequences is less pronounced than in the human and mouse genomes |
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Authors: | P D Thomsen J R Miller |
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Institution: | (1) Division of Anatomy, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark, DK;(2) The Babraham Institute, Laboratory of Genome Mapping, Department of Immunology, Babraham Hall, Cambridge CB2 4AT, UK, GB |
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Abstract: | The distribution of SINE and LINE sequences in the pig genome was examined by fluorescence in situ hybridization (FISH),
interspersed repeat PCR, and restriction analysis of high molecular weight DNA. FISH revealed a largely uniform hybridization
to the euchromatic chromosome regions with both interspersed repeats, although a bias toward the G-bands was observed for
the LINE probe. Southern blots of inter-SINE and inter-LINE PCR products showed strong hybridization to LINE and SINE probes,
respectively. High molecular weight DNA derived from a pig |m~ hamster hybrid cell line was cut with a panel of G + C and
A + T rich rare cutter restriction enzymes, then run on a pulsed field gel and Southern blotted. Sequential hybridization
with SINE and LINE probes showed that SINE hybridization was to relatively low molecular weight fragments with the G + C rich
enzymes, whereas the LINE probe gave hybridization to significantly larger fragments produced by these enzymes. DNA samples
digested with A + T rich enzymes gave essentially similar patterns with SINE and LINE probes. We conclude that the pattern
of differential distribution of SINEs and LINEs, which has been described in man and mouse, does exist in the pig but is much
less pronounced.
Received: 25 April 1995 / Accepted: 1 September 1995 |
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