Sequence Analysis of the Bacillus subtilis 168 Chromosome Region between the sspC and odhA Loci (184{degrees}-180{degrees}) |
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| Authors: | Ghim, Sa-Youl Choi, Soo-Keun Shin, Byung-Sik Jeong, Young-Mee Sorokin, Alexei Ehrlich, S. Dusko Park, Seung-Hwan |
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| Affiliation: | 1Bacterial Molecular Genetics RU, Korea Research Institute of Bioscience and Biotechnology (KRIBB) P.O. Box 115, Yusong, Taejon 305-600, Korea
2Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique Domaine de Vilvert, 78352 Jouy en Josas cedex, France |
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| Abstract: | The nucleotide sequence of 45,389 bp in the 184°-;180°region of the Bacillus subtilis chromosome, containing the cgecluster, which is controlled by the sporulation regulatory proteinGerE, was determined. Fifty-four putative ORFs with putativeribosome-binding sites were recognized. Seven of them correspondto previously characterized genes: cgeB, cgeA, cgeC, cgeD, cgeE,ctpA, and odhA. The deduced products of 25 ORFs were found todisplay significant similarities to proteins in the data banks.We have identified genes involved in detoxification, cell walls,and in the metabolism of biotins, purines, fatty acids, carbohydratesand amino acids. The remaining 22 ORFs showed no similarityto known proteins. Both an attachment site of the SPßprophage and 2 new putative DNA replication terminators wereidentified in this region. |
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| Keywords: | Bacillus subtilis genome sequencing sspC-odhA region |
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