Contribution of glycolipids to species-specific antigens on erythrocytes of several animal species as to recognition of antigens with rabbit anti-glycolipids and anti-erythrocyte antisera |
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Authors: | Masao Iwamori Makoto Murata Mariko Toyoda Yuriko Iwamori |
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Institution: | (1) Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka Osaka, 577-8502, Japan |
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Abstract: | Because anti-glycolipid antibodies are involved in the onset of several neurological diseases, the reactivity of glycolipids
on erythrocytes and the probability of generating the antibodies were determined to clarify the contribution of glycolipids
as antigens. Anti-erythrocyte antisera reacted with the following glycolipids in a species-specific manner, i.e. blood group A-active glycolipid for man, Forssman glycolipid for sheep, Gg3Cer for guinea pig, and Gg4Cer and fucosyl GM1 for rat, and the hemolytic activities of the anti-erythrocyte antisera were attenuated by absorption of
the antisera with liposomes prepared from the lipids of erythrocytes to the following levels, 94.5% for man, 24.5% for sheep,
17.5% for guinea pig, and 54.5% for rat. These species-specific glycolipids on erythrocytes reacted well with the respective
anti-glycolipid antisera, but Gb4Cer in man and GM1 in rat were shown to be cryptic on immunization with erythrocytes, indicating that the contribution of
glycolipids as erythrocyte antigens differs among animal species.
The glycolipid nomenclature is based on the recommendations of the IUPAC-IUB Commission on Biochemical Nomenclature 1]. The ganglioside nomenclature of Svennerholm is employed throughout 2]. |
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Keywords: | Anti-glycolipid antisera Liposomes TLC-immunostaining Hemolytic inhibition Species-specificity Glycosphingolipids |
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