Determination of chemical biodegradation by direct and indirect methods |
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Authors: | Dinesh D Vaishnav Eric T Korthals |
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Institution: | (1) Mid-State Associates, Baraboo, WI, U.S.A.;(2) AScI-Duluth Environmental. Testing Division, AScI Corporation, 112 East Second Street, 55805 Duluth, MN, U.S.A. |
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Abstract: | Summary Degradation of 10 organic chemicals by pre-acclimated microorganisms in BOD dilution water was determined directly by UV spectrophotometry and indirectly by a modified BOD method. Residual chemical concentrations were periodically measured and pseudo-first-order biodegradation rate constants (k
1) were calculated. Thek
1 spectrophotometry values ranged from 0.006/h to 0.077/h andk
1-BOD values from 0.002/h to 0.043/h for 1-methylnaphthalene and indole, respectively. The ratios ofk spectrophotometry to k1-BOD were between 1.5 for salicylic acid and 3.0 for 1-methylnaphthalene with a mean of 2.7. A significant ( =0.001) linear correlation (r
2=0.854,F=46.630) existed between the two sets of rate constants. Results from this study suggest that the modified BOD method may be used to estimate chemical biodegradation rates in synthetic media. |
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Keywords: | Biodegradation Direct method Indirect method Method comparison BOD method |
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