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Subcellular Distribution of Aspartate Transaminase,Alanine Amino Transferase,Glutamate Dehydrogenases,and Lactate Dehydrogenase in Tetrahymena* |
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| Authors: | PAMELA PORTER J J BLUM H ELROD |
| Abstract: | SYNOPSIS. Mitochondria and peroxisomes were isolated from homogenates of Tetrahymena pyriformis by sedimentation through a sucrose gradient. Succinate dehydrogenase was used as a mitochondrial marker; catalase and isocitrate lyase were used to mark the peroxisomal fraction. Lactate dehydrogenase, glutamate dehydrogenase, and alanine aminotransferase were found only in the mitochondrial fraction. Aspartate transaminase was found in both mitochondrial and peroxisomal fractions. |
| Keywords: | Tetrahymena pyriformis mitochondria peroxisomes sucrose gradient sedimentation succinate dehydrogenase catalase isocitrate lyase lactate dehydrogenase glutamate dehydrogenase alanine aminotransferase aspartate transaminase |