Inheritance Characteristics of Microsatellite DNA Loci in Experimental Families of Japanese Flounder Paralichthys olivaceus |
| |
Authors: | Masashi Sekino Motoyuki Hara |
| |
Institution: | (1) Tohoku National Fisheries Research Institute, Shinhama, Shiogama, Miyagi, 985-0001, Japan, JP;(2) National Research Institute of Aquaculture, Nansei, Watarai, Mie, 516-0193, Japan, JP |
| |
Abstract: | Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring
errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele.
At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype
frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information
supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.
Received September 25, 2000; accepted March 1, 2001 |
| |
Keywords: | : microsatellite DNA loci Japanese flounder Paralichthys olivaceus Mendelian inheritance |
本文献已被 PubMed SpringerLink 等数据库收录! |
|