Spontaneous <Emphasis Type="Italic">in vitro</Emphasis> Hybridization of LDH Homopolymers in the Undenatured State

UNTIL now, there has been no totally satisfactory hypothesis proposed for the hybridization of lactate dehydrogenase in vivo, This is because hybridization in vitro, the only experimental approach, has usually been accomplished by chaotropic agents: freeze-thaw¹, guanidine^{2, 3}, urea³, or acid denaturation⁴. Whatever means the cell uses, it follows^5–8 that it is susceptible to environmental influences. We now describe a gentle hybridization between heart and muscle types which can occur in more physiological conditions and which mimics many observations in vivo.