RNA partitioning accompanied by adsorption: high-molecular-mass RNA adsorbed at the interface like a particle |
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Authors: | K. Kimura and H. Kobayashi |
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Affiliation: | Bioengineering Department, National Institute of Bioscience and Human-Technology, Higashi 1-1, Tsukuba, Ibaraki 305, Japan |
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Abstract: | In a potassium phosphate-poly(ethylene glycol) (PEG) system, RNA partitioning was accompanied by adsorption at the interface, depending on the molecular mass. Low-molecular-mass RNA showed the typical partition behavior of a soluble substance. Conversely, high-molecular-mass RNA was significantly adsorbed at the interface in the potassium phosphate-PEG1500 system. The adsorbed amount was proportional to the added amount, regardless of the phase volume ratio. The partitioning of high-molecular-mass RNA showed an amount-ratio-dependent distribution like that of a particle. |
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Keywords: | Partitioning RNA Nucleic acids |
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