PACAP activates PKA, PKC and Ca(2+) signaling cascades in rat neuroepithelial cells. |
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| Authors: | C J Zhou T Yada D Kohno S Kikuyama R Suzuki H Mizushima S Shioda |
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| Institution: | Department of Biology, School of Education, Waseda University, Nishi-Waseda 1-6-1, Shinjuku-ku, Tokyo 169-8050, Japan. |
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| Abstract: | Several studies have reported that the PAC(1) receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca(2+) concentration (Ca(2+)](i)) was increased by PACAP, but not VIP, in a concentration range from 10(-12) to 10(-8) M via the PAC(1)-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC(1)-R is linked to Ca(2+)](i) signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment. |
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