Lymphocyte stimulation by allogeneic and autochthonous cultured lymphoid cells |
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Authors: | J S Flier P R Glade S W Broder K Hirschhorn |
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Affiliation: | 1. Division of Rheumatology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY, USA;2. Division of Rheumatology, Hackensack University Medical Center, Hackensack, NJ, USA;3. Department of Medicine, Jacobi Medical Center, Bronx, NY, USA;4. Department of Medicine, University of Wisconsin Hospital and Clinics, WI, USA;5. Department of Medicine, University of Connecticut School of Medicine, CT, USA;6. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA;7. Department of Genetics, Albert Einstein College of Medicine, Bronx, NY USA;8. Department of Oncology and Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY USA;9. Division of Nephrology, Ohio State University Wexner Medical Center, Columbus, Ohio, USA;10. Division of Rheumatology, Department of Medicine, University of Michigan, MI, USA;1. Knowledge Hub Aichi, Aichi Science and Technology Foundation, 1267-1 Akiai, Yakusa cho, Toyota 470-0356, Japan;2. Graduate School of Bioagricultural Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan |
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Abstract: | The ability of allogeneic and autochthonous lymphoid cells maintained in long-term culture and three-day cultured PHA-stimulated autochthonous lymphocytes to stimulate fresh peripheral lymphocytes was investigated via the one-way mixed lymphocyte test. Long-term lymphoid cell lines stimulated fresh allogeneic peripheral lymphocytes as measured by DNA incorporation. Although autochthonous lines were usually not stimulatory, several experiments indicate that fresh peripheral lymphocytes may be stimulated by autochthonous long-term lines and phytohemagglutinin-transformed autochthonous lymphocytes. The major histocompatibility antigens were identical in fresh donor cells and autochthonous long-term lymphoid cell lines as demonstrated by standard cytotoxicity methods. Although the reason for this stimulation is not certain, the most likely possibility is the production of a blastogenic factor by these cell systems. |
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