P2X7 receptor involved in antitumor activity of atractylenolide I in human cervical cancer cells |
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Authors: | Yue Han Can Bai Xi-Meng He Qing-Ling Ren |
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Affiliation: | 1.Department of Gynecology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029 China ;2.Acupuncture & Chronobiology Key Laboratory of Sichuan Province, Chengdu University of Traditional Medicine, Chengdu, 610075 China |
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Abstract: | Atractylenolide I (Atr-I) was found to sensitize a variety of human cancer cells in previous studies. Purinergic P2X7R plays important role in different cancers. However, whether Atr-I could generate antitumor activity in human cervical cancer cells and P2X7R get involved in this effect remain unclear. In this study, Hela (HPV 18 +) and SiHa (HPV 16 +) cells were treated with different doses of Atr-I. The results indicated that agonist and antagonist of P2X7 receptors, BzATP and JNJ-47965567 (JNJ), could suppress the proliferation of Hela and SiHa cells. Atr-I demonstrated a considerable antitumor effect in both human cervical cancer cells in vitro. Atr-I combined with P2X7R agonist, BzATP, restored Atr-I-induced growth inhibition in Hela cells but not in SiHa cells. However, the combinatorial treatment of P2X7R antagonist JNJ and Atr-I has an additive effect on cell growth inhibition in SiHa cells rather than in Hela cells. It implied that P2X7R would get involved in the anti-human cervical cancer cells effect of Atr-I. |
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Keywords: | Atractylenolide I P2X7Rs Hela cells SiHa cells Human cervical cancer cells |
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