Heterotrophic plate counts and the isolation of bacteria from mineral waters on selective and enrichment media |
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Authors: | C M Manaia O C Nunes P V Morais M S da Costa |
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Institution: | Departamento de Zoologia, Universidade de Coimbra, Portugal. |
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Abstract: | The heterotrophic plate counts of 15 brands of bottled non-carbonated mineral waters were examined and found to be generally high and variable. Four selective or enrichment media for the enumeration of coliforms (m-Endo LES and m-lauryl sulphate agar) and Pseudomonas aeruginosa (cetrimide-nalidixic acid agar and malachite green broth) were used to isolate several species of Gram-negative bacteria. Strains identified as CDC gr IVc-2 and Comamonas (Ps.) acidovorans were the two most commonly isolated. Considerable variation in populations was seen between the brands, as well as between two batches of the same mineral water. |
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